ESTERIFICATION OF GLYCOSIDES BY A MONOACYLGLYCEROL AND DIACYLGLYCEROLLIPASE FROM PENICILLIUM-CAMEMBERTII AND COMPARISON OF THE PRODUCTS WITH CANDIDA-CYLINDRACEA LIPASE

Glycosides with glycerol and trimethylolpropane (TMP) moieties at the reducing ends were esterified by mono- and diacylglycerol lipase from Penicillium camembertii. Several TMP glycosides were esterified to a g reater extent than those of glycerol. The products from glucosyl TMP a nd oleic acid by Penicillium and Candida cylindracea lipases were isol ated and their structures elucidated. The lipases formed two monoester s; a major monoester whose TMP moiety was esterified and a minor monoe ster bearing an oleic acid group at the glucose moiety. One of the two diesters has a diacylated TMP moiety, while the other has acyl groups at both TMP and glucose moieties. Candida lipase formed these diester s in an almost equal ratio. Penicillium lipase formed the latter one p referentially. Candida lipase synthesized tri- and tetraesters, which accounted for about 34% of all the products, while Penicillium lipase formed these highly esterified products at a level of only 4 % of the total products.