PHASE-SENSITIVE 2-DIMENSIONAL EXPERIMENT ON LIVING ANIMALS

This report describes the first H-1-H-1 phased spectrum obtained in vi vo from the hind-leg muscles of an intact mouse. TOCSY correlations ca n follow the complete spin system and give a more detailed graph of ea ch molecule than can COSY. TOCSY is also better suited than COSY for s tudying long fatty-acid chains. All the peaks are in phase in a TOCSY experiment and the intensities of the cross-correlation peaks are less sensitive to low digitalization in the t(1) domain than are those of COSY. The improvement in sensitivity was estimated by measuring the vo lumes of the cross-correlation peaks in COSY and TOCSY spectra. (C) 19 95 Academic Press, Inc.