Pm. Girard et al., A SHORT AUTOCOMPLEMENTARY SEQUENCE IN THE 5'-LEADER REGION IS RESPONSIBLE FOR DIMERIZATION OF MOMULV GENOMIC RNA, Biochemistry, 34(30), 1995, pp. 9785-9794
Previous work has shown that a region of Moloney murine leukemia virus
(MoMuLV) RNA located between nucleotides 280 and 330 in the PSI regio
n (nt 215-565) is implicated in the dimerization process. We show with
a deletion from nucleotides 290-299 in PSI RNA transcripts and throug
h an antisense oligonucleotide complementary to nucleotides 275-291 th
at the 283-298 region is involved in RNA dimer formation in vitro. In
an attempt to further characterize the mechanism of dimer formation, a
series of short RNA transcripts was synthesized which overlapps the P
SI region of MoMuLV RNA. The dimerization of these RNAs is temperature
dependent. The predicted secondary structure of the 278-303 region, a
s a function of temperature, reveals that this sequence is able to ado
pt two conformations: (1) the U(288)AGCUA(293) sequence in a loop (2)
part of the same nucleotides implicated in a stem. These results, toge
ther with thermodynamic analysis, strongly suggest that (1) the loop c
onformation of the UAGCUA sequence modulates the relative amount of RN
A dimer and (2) a 16 bp long Watson-Crick base pairing is involved in
RNA dimer formation. We propose that loop-loop recognition via the U(2
88)AGCUA(293) sequence leads to a stable structure induced by a stem-l
oop opening. Furthermore, our results do not support purine quartet fo
rmation as necessary for the dimerization of the 5' leader MoMuLV RNA.