SIMULTANEOUS PROFILE ANALYSIS OF PLASMA AMINO AND ORGANIC-ACIDS BY CAPILLARY GAS-CHROMATOGRAPHY

Authors
HUSEK P
Citation
P. Husek, SIMULTANEOUS PROFILE ANALYSIS OF PLASMA AMINO AND ORGANIC-ACIDS BY CAPILLARY GAS-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 669(2), 1995, pp. 352-357
Citations number
11
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
669
Issue
2
Year of publication
1995
Pages
352 - 357
Database
ISI
SICI code
Abstract
A simultaneous GC analysis of more than 20 amino and nearly 30 non-ami no organic acids abundant in plasma is for the first time possible. Is olation of the analytes from the plasma matrix is not necessary, keto acids do not require a preliminary oximation. An instantaneous derivat ization of the acids with ethyl chloroformate takes place directly in the medium after deproteinization. Less than 30 min are required to pr epare a plasma sample for the GC analysis.