P. Husek, SIMULTANEOUS PROFILE ANALYSIS OF PLASMA AMINO AND ORGANIC-ACIDS BY CAPILLARY GAS-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 669(2), 1995, pp. 352-357
Citations number
11
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A simultaneous GC analysis of more than 20 amino and nearly 30 non-ami
no organic acids abundant in plasma is for the first time possible. Is
olation of the analytes from the plasma matrix is not necessary, keto
acids do not require a preliminary oximation. An instantaneous derivat
ization of the acids with ethyl chloroformate takes place directly in
the medium after deproteinization. Less than 30 min are required to pr
epare a plasma sample for the GC analysis.