SIMULTANEOUS DETERMINATION OF THEOPHYLLINE, ENOXACIN AND CIPROFLOXACIN IN HUMAN PLASMA AND SALIVA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
ZHAI SP KORRAPATI MR WEI XX MUPPALLA S VESTAL RE
Citation
Sp. Zhai et al., SIMULTANEOUS DETERMINATION OF THEOPHYLLINE, ENOXACIN AND CIPROFLOXACIN IN HUMAN PLASMA AND SALIVA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 669(2), 1995, pp. 372-376
Citations number
9
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
669
Issue
2
Year of publication
1995
Pages
372 - 376
Database
ISI
SICI code
Abstract
A simple reversed-phase high-performance liquid chromatographic method has been developed for the simultaneous determination of theophylline , ciprofloxacin and enoxacin in plasma and saliva. The biological flui d samples were extracted with methylene chloride-isopropyl alcohol pri or to isocratic chromatography on a Waters C-18 mu Bondapak column. Ul traviolet detection was carried out at 268 nm. The assay is linear for ciprofloxacin and enoxacin (0.05-10 mu g/ml), and theophylline (0.1-2 0 mu g/ml). The assay can be used to investigate the interaction of th ese two fluoroquinolones with theophylline.