IMPROVED CLEANUP PROCEDURE FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF CLOMIPRAMINE AND ITS DEMETHYLATED METABOLITE IN HUMAN PLASMA

Authors
ALTIERI I PICHINI S PACIFICI R ZUCCARO P
Citation
I. Altieri et al., IMPROVED CLEANUP PROCEDURE FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF CLOMIPRAMINE AND ITS DEMETHYLATED METABOLITE IN HUMAN PLASMA, Journal of chromatography B. Biomedical applications, 669(2), 1995, pp. 416-417
Citations number
1
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
669
Issue
2
Year of publication
1995
Pages
416 - 417
Database
ISI
SICI code
Abstract
A rapid and selective assay of clomipramine and its metabolite desmeth ylclomipramine in human plasma, based on high-performance liquid chrom atography with UV detection has been developed. The compounds were sub jected to solid-phase extraction, using Extrelut 1 cartridges. Recover ies ranged between 88-95% for clomipramine, and 75-80% for desmethylcl omipramine. This method has been used for therapeutic monitoring of cl omipramine and its metabolite in individuals treated with this drug.