SYNTHESIS AND ANTITUMOR-ACTIVITY OF ISO-P IRITREXIM, A LIPOPHILIC FOLATE ANTAGONIST

Two strategies rewards the synthesis of Iso-Piritrexim (12) are descri bed. A) The Mannich-reaction of ketone 2 yields the bases 4-HCl and 5- HCl. By means of LC base 4 is separated and treated with in situ gener ated 3,3-diaminoacrylonitrile (9) to yield the 2-aminonicotinonitrile 11. The cyclocondensation of 11 with guanidine provides Iso-PTX (12). B) Reduction and oxidation of the beta-ketoester 15 leads to the beta- ketoaldehyde 17, which is cyclocondensed with 2,4,6-triaminopyrimidine (18) to yield Iso-PTX (12). In the NCl-tumor-test Iso-PTX (12) shows a moderate activity against some leukemia and lung cancer cell lines.