LOCAL CA2+ RELEASE FROM INTERNAL STORES CONTROLS EXOCYTOSIS IN PITUITARY GONADOTROPHS

Exocytosis and the cell-averaged cytosolic [Ca2+], [Ca2+](i), were tra cked in single gonadotrophs. Cells released 100 granules/s at 1 mu M = [Ca2+](i) when gonadotropin-releasing hormone (GnRH) activated IP3-me diated Ca2+ release from internal stores, but only 1 granule/s when [C a2+](i) was raised uniformly to 1 mu M by other means. Strong exocytos is was then seen only at higher [Ca2+](i) (half-maximal at 16 mu M). P arallel second messengers did not contribute to GnRH-induced exocytosi s, because IP3 alone was as effective as GnRH, and because even GnRH f ailed to trigger rapid exocytosis when the [Ca2+](i) rise was blunted by EGTA. When [Ca2+](i) was released from stores, exocytosis depended on [Ca2+](i) rising rapidly, as if governed by Ca2+ flux into the cyto sol. We suggest that IP3 releases Ca2+ selectively from subsurface cis ternae, raising [Ca2+] near exocytic sites B-fold above the cell avera ge.