EXPRESSION OF HUMAN TYPE-I INTERLEUKIN-8 RECEPTOR CDNA IN FIBROBLASTSBALB 3T3 AND CHARACTERIZATION OF THE EXPRESSION PRODUCTS

Authors
TIKHONOV II FOMIN IK DOROSHENKO TM CHALYI YV VOITENOK NN
Citation
Ii. Tikhonov et al., EXPRESSION OF HUMAN TYPE-I INTERLEUKIN-8 RECEPTOR CDNA IN FIBROBLASTSBALB 3T3 AND CHARACTERIZATION OF THE EXPRESSION PRODUCTS, Molecular biology, 30(5), 1996, pp. 600-604
Citations number
24
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
30
Issue
5
Year of publication
1996
Part
1
Pages
600 - 604
Database
ISI
SICI code
0026-8933(1996)30:5<600:EOHTIR>2.0.ZU;2-6
Abstract
We expressed the cDNA of human interleukin-8 (IL-8) receptor type I in murine BALE 3T3 fibroblasts using a retrovirus vector. The properties of the receptor thus produced and the IL-8-binding proteins of human neutrophils were compared. Two IL-8-binding proteins of 70 and 45-47 k Da were revealed in fibroblasts constitutively expressing the membrane receptor. Three major IL-8-binding proteins of 70, 58, and 45-47 kDa were revealed in human neutrophils. Since the molecular mass of type I I IL-8 receptor is 45 kDa, the origin of the 58-kDa protein is unclear .