RAPID AND DIFFERENTIAL DETECTION OF 2 ANALOGOUS ENTEROTOXINS OF VIBRIO-CHOLERAE AND ENTEROTOXIGENIC ESCHERICHIA-COLI BY A MODIFIED ENZYME-LINKED-IMMUNOSORBENT-ASSAY

Authors
KOIKE N OKADA K YABUSHITA Y ZHANG DY YAMAMOTO K MIWATANI T HONDA T
Citation
N. Koike et al., RAPID AND DIFFERENTIAL DETECTION OF 2 ANALOGOUS ENTEROTOXINS OF VIBRIO-CHOLERAE AND ENTEROTOXIGENIC ESCHERICHIA-COLI BY A MODIFIED ENZYME-LINKED-IMMUNOSORBENT-ASSAY, FEMS immunology and medical microbiology, 17(1), 1997, pp. 21-25
Citations number
14
Categorie Soggetti
Immunology,Microbiology
Journal title
FEMS immunology and medical microbiology → ACNP
ISSN journal
09288244
Volume
17
Issue
1
Year of publication
1997
Pages
21 - 25
Database
ISI
SICI code
0928-8244(1997)17:1<21:RADDO2>2.0.ZU;2-F
Abstract
The principle of a novel ELISA (nylon-slip immune-test, NSIT) was appl ied to the differential detection of two analogous enterotoxins, chole ra toxin (CT) of Vibrio cholerae and heat-labile enterotoxin (LT) of e nterotoxigenic Escherichia coli. The results obtained for CT and LT de tection by a single test were sufficiently sensitive (87.9 and 100%) a nd specific (100 and 94.7%) in the differential detection test, when c ompared with the result of a colony hybridization test with DNA probes . The results suggest that the novel ELISA is applicable to the diagno sis of bacterial infections, by means of differential immunological de tection of toxins in a single test.