Y. Chu et Rj. Hurtubise, COMPARISON OF THE SOLID-MATRIX LUMINESCENCE PROPERTIES OF BENZO(A)PYRENE-DNA ADDUCTS ON ALPHA-CYCLODEXTRIN NACL AND TREHALOSE/NACL MATRICES/, Photochemistry and photobiology, 62(2), 1995, pp. 251-257
The solid-matrix luminescence properties of i-9,10-epoxy-7,8,9,10-tetr
ahydrobenzo(a)pyrene-DNA ([+/-]-anti-BPDE-DNA) adducts were compared o
n alpha-cyclodextrin (CD)/NaCl and trehalose/NaCl solid matrices. Both
the optimum composition for the solid matrices and the best solvent s
ystem were obtained experimentally for acquiring the maximum room-temp
erature fluorescence (RTF) and room-temperature phosphorescence (RTP)
signals for the (+/-)-anti-BPDE-DNA. Most of the solid-matrix RTF and
RTP data were obtained at 296 K and 93 K for (+/-)-anti-BPDE-DNA adduc
ts adsorbed on 1% alpha-CD/NaCl and 80% trehalose/NaCl. The RTF signal
s were strong for (+/-)-BPDE-DNA adducts on both solid matrices, but R
TP was only obtained on the trehalose/NaCl solid matrices with the 80%
trehalose yielding the strongest RTP signal for (+/-)-anti-BPDE-DNA.
The fluorescence lifetime data for (+/-)-anti-BPDE-DNA gave two compon
ents on 1% alpha-CD/NaCl. For 80% trehalose/NaCl, three components wer
e revealed, but two components were obtained with 80% trehalose/NaCl a
fter ether extraction of the solid matrix. The third component was asc
ribed to the formation of the tetrols from (+/-)-anti-BPDE-DNA adducts
during the drying step in the sample preparation of 80% trehalose/NaC
l. The results give the first reported data on the solid-matrix lumine
scence of the (+/-)-anti-BPDE-DNA adducts. These results should be of
considerable interest not only from an analytical viewpoint but as a n
ew means of studying the luminescence characteristics of the adducts.