MAPPING CELLS AND SUBCELLULAR ORGANELLES ON 2-D GELS - NEW TRICKS FORAN OLD HORSE

Nowadays, investigators in all fields are faced with the identificatio n of unknown, up- or down-regulated, modified proteins that they are t rying to identify. Two-dimensional (2-D) gel electrophoresis, with its ability to resolve several thousand proteins, is an extremely powerfu l technique. The current resolution and reproducibility of 2-D gel tec hnology and the establishment of computer assisted 2-D gel protein dat abases have paved new ways for the identification of proteins.