INTEGRATED MOLECULAR-SYSTEMS FOR BIOSENSORS

Redox enzymes have been assembled in a monolayer on a solid surface by a potential-assisted self-assembly method as well as a thiol-gold sel f-assembly method. These enzymes communicate electronically with the s olid substrate through a molecular interface conducting polymer and a covalently bound mediator. An ordered antibody array has also been ass embled on the solid surface by a combination of the Langmuir-Blodgett (LB) film method and the self-assembly method. An ordered monolayer of protein A is deposited on the solid surface by the LB method, which i s followed by self assembly of antibody. Individual antigen molecules that are complexed with the antibody array have been quantitated selec tively by atomic force microscopy (AFM), A TOL plasmid, encoding a xyl R binding protein for xyline and a firefly luciferase marker enzyme, has been implemented in a bacterial cell. The whole cell responds to e nvironmentally hazardous substances such as xylene by emitting light.