SELF-ASSEMBLY OF MEDIATOR-MODIFIED ENZYME IN POROUS GOLD-BLACK ELECTRODE FOR BIOSENSING

Ferrocene-modified glucose oxidase, in which 6-11 ferrocene molecules are covalently attached to glucose oxidase, is covalently bound to sel f-assembled aminoethane thiol on a smooth gold electrode surface or a porous gold-black electrode surface. Reversible electron transfer is d emonstrated between the ferrocene-modified glucose oxidase and the ele ctrode. Enzymatic oxidation of glucose is efficiently coupled with the electrochemical oxidation of enzyme through the enzyme-bound mediator . The electron transfer of modified enzyme is enhanced by the gold-bla ck electrode, which can provide the modified enzyme with increased ele ctron-transfer sites.