EXPERIMENTAL INVESTIGATIONS ON THE IMMUNOMODULATING ACTIVITY OF POLYBACTERIAL PREPARATION FOR PERORAL IMMUNOTHERAPY AND IMMUNOPROPHYLAXIS OF UROINFECTIONS

The immunomodulating effect of a polybacterial immunostimulator Urosti m in experimental animal models was investigated. The preparation was created for immunotherapy and immunoprophylaxis of uroinfections. It c onsists of killed bacterial cells and their lysates of four microbial species: Escherichia coli expressing type I pill, Rc mutant of E. coli , Klebsiella pneumoniae, Proteus mirabilis and Enterococcus faecalis, BALB/c mice and guinea-pigs were treated orally with Urostim for 5 or 10 days, respectively. A stimulatory effect of Urostim on phagocytosis (increased phagocytic index and phagocytic number) of mice peritoneal exudate cells was observed. An increased number of plaque-forming cel ls and elevated titres of haemagglutinating antibodies in mice demonst rated activation of humoral immunity. Lymphoproliferative studies show ed a significant response to phytohaemagglutinin (PHA) and Urostim, es pecially after the second application of the preparation. At the same time no changes in the absolute number of peripheral blood lymphocytes were found. An active protection of mice against a systemic infection and of guinea-pigs against uroinfection caused by Gram-negative bacte ria was obtained. In conclusion, the results obtained characterize Uro stim as an effective immunostimulator. Its peroral application leads t o the activation of innate resistance, cell-mediated, humoral and loca l immunity.