SIMULTANEOUS DETECTION BY CONSENSUS MULTIPLEX PCR OF HIGH-RISK AND LOW-RISK AND OTHER TYPES OF HUMAN PAPILLOMA-VIRUS IN CLINICAL-SAMPLES

A consensus multiplex PCR (CM-PCR) technique was developed to detect h igh-risk (HPV 16/18), low-risk (HPV 6/11), and over 40 other types of human papillomavirus (HPV), separately but simultaneously, by mixing t hree pairs of consensus primers in the same PCR mixture, for gene ampl ification. Simultaneous detection of three groups of HPV DNA provides valuable information for clinical practice and this procedure is simpl e and convenient for routine laboratory examinations. We detected HPV DNA sequences in plasmid HPV DNA and DNA extracted from tissues of con dyloma acuminata and cervical carcinoma and from exfoliated cells of t he lower genital tract of healthy Chinese women living in the People's Republic of China. We confirmed that this simple, convenient, and cos t-beneficial CM-PCR technique is reliable for the detection of HPV DNA sequences. (C) 1995 Academic Press, Inc.