THE HYPOXIC RESPONSE OF 3 ALCOHOL-DEHYDROGENASE GENES - IN-VIVO AND IN-VITRO FOOTPRINTING OF DNA PROTEIN INTERACTIONS DESCRIBES MULTIPLE SIGNALING CONNECTIONS/

The induction of plant genes by hypoxia has served as a paradigm for u nderstanding gene expression in response to environmental stresses. We have used multiple approaches to our study of gene induction by hypox ia, beginning with in vitro footprinting of alcohol dehydrogenase (adh ) genes as they enter and maintain active transcription in response to reduced environmental oxygen. These experiments, combined with the mo lecular dissection of promoters and studies on chromatin structure, ha ve lead to a derailed description of the molecular state of adh genes in a transcriptionally active mode. Recently, we have cloned some of t he protein factors involved in regulating the expression of adh genes, and have begun to understand the possible associations that exist amo ng gene regulatory proteins and diverse cell signalling pathways. (C) 1997 Annals of Botany Company