ANION-EXCHANGE HPLC ANALYSIS OF BIOTINYLATED OLIGONUCLEOTIDES

Biotinylated oligonucleotides combined with streptavidin-coated magnet ic bends are commonly used in current molecular biology. Their quality and the level of incorporated biotin are essential for yielding good results in either solid-phase DNA sequencing or solid-phase purificati on procedures. This paper presents a very simple analytical test using anion-exchange HPLC and avidin to ascertain the quality of biotinylat ed oligonucleotides and to predetermine their ability to bind to avidi n, which is a prerequisite for functionality in some solid-phase metho ds.