RAPID MICROPLATE MITOGENIC ASSAY USING A MELTABLE SCINTILLATION WAX

To combine the advantages of solid scintillation and the use of 96-wel l plates, an in-plate cell proliferation assay was designed using scin tillation wax. Messy, conventional liquid wastes are not produced, the reby making solid scintillation safer and more convenient. Additionall y the use of optimized, 96-well ViewPlates(TM) for inplate counting pr ocedures allows the monitoring of cell morphology during the course of -the stimulation experiment. The scintillation was; FlexiScint(TM), wa s used for counting [H-3]thymidine incorporation in an adherent cell l ine and compared to liquid scintillation counting. The data presented here demonstrate that this new assay produces results comparable to th ose obtained by conventional methods.