LARGE-SCALE GENOMIC SEQUENCING - OPTIMIZATION OF GENOMIC CHEMICAL SEQUENCING REACTIONS

We have developed a streamlined reproducible method for performing gen omic chemical sequencing reactions on the genomic DNA of Mycoplasma ca pricolum, which has a genome size of about 750000 base pairs and whose composition is 75% AT The general modifications that ensure reproduci bility and allow the processing of multiple samples can be widely adop ted to other large-scale sequencing projects, while the specific modif ications to the chemical reactions are applicable to the sequencing of other DNAs with a high AT content.