DEVELOPMENT OF A NOVEL TNF-ALPHA LIGAND-RECEPTOR BINDING ASSAY FOR SCREENING NATCHEM(TM) LIBRARIES

Authors
MACALLAN D SOHAL J WALKER C HILL D MOORE M
Citation
D. Macallan et al., DEVELOPMENT OF A NOVEL TNF-ALPHA LIGAND-RECEPTOR BINDING ASSAY FOR SCREENING NATCHEM(TM) LIBRARIES, Journal of receptor and signal transduction research, 17(1-3), 1997, pp. 521-529
Citations number
24
Categorie Soggetti
Cell Biology",Biology
Journal title
Journal of receptor and signal transduction research → ACNP
ISSN journal
10799893
Volume
17
Issue
1-3
Year of publication
1997
Pages
521 - 529
Database
ISI
SICI code
1079-9893(1997)17:1-3<521:DOANTL>2.0.ZU;2-Z
Abstract
The TNF alpha receptor is a major therapeutic target since over produc tion of TNF alpha plays a key role in the development of septic shock following bacterial infection and has been implicated in the pathogene sis of many inflammatory disorders such as rheumatoid arthritis. To sc reen our NATCHEM(TM) Libraries for novel natural product inhibitors of this target we have developed a sensitive immobilised TNF alpha recep tor binding assay based on a commercially available recombinant solubl e TNF alpha receptor(p55) and [I-125]-TNF alpha.