INCREASED TITER OF RECOMBINANT AAV VECTORS BY GENE-TRANSFER WITH ADENOVIRUS COUPLED TO DNA-POLYLYSINE COMPLEXES

One of the principal problems with using recombinant adeno-associated virus vectors (rAAV) as vehicles for gene delivery has been the diffic ulty in obtaining high-titer virus stocks after the initial transfecti on into producer cells. In this report we describe a method for transf ecting cells at extremely high efficiency with rAAV vector DNA and com plementation plasmid while simultaneously infecting those cells with r eplication competent adenovirus using adenovirus-polylysine-DNA comple xes. We further show that this technique results in an increase in rAA V transducing titer by two orders of magnitude over what is typically achieved by standard calcium phosphate transfection.