C-13-NMR SPECTROSCOPY OF KERATAN SULFATES - ASSIGNMENTS FOR 4 POLY(N-ACETYLLACTOSAMINE)-REPEAT-SEQUENCE TETRASACCHARIDES DERIVED FROM BOVINE ARTICULAR-CARTILAGE KERATAN SULFATE BY KERATANASE-II DIGESTION

Authors
HUCKERBY TN BROWN GM NIEDUSZYNSKI IA
Citation
Tn. Huckerby et al., C-13-NMR SPECTROSCOPY OF KERATAN SULFATES - ASSIGNMENTS FOR 4 POLY(N-ACETYLLACTOSAMINE)-REPEAT-SEQUENCE TETRASACCHARIDES DERIVED FROM BOVINE ARTICULAR-CARTILAGE KERATAN SULFATE BY KERATANASE-II DIGESTION, European journal of biochemistry, 231(3), 1995, pp. 779-783
Citations number
16
Categorie Soggetti
Biology
Journal title
European journal of biochemistry → ACNP
ISSN journal
00142956
Volume
231
Issue
3
Year of publication
1995
Pages
779 - 783
Database
ISI
SICI code
0014-2956(1995)231:3<779:CSOKS->2.0.ZU;2-3
Abstract
Skeletal keratan sulphate has been fragmented using the enzyme keratan ase II, and the complete C-13 chemical shift data are reported for the four tetrasaccharides which derived from the repeat region. They have the structures: Gal beta(1-4)GlcNAc(6S)beta(1-3)Gal beta(1-4)GlcNAc(6 S)-ol, beta(1-4)GlcNAc(6S)beta(1-3)Gal(1-4)GlcNAc(6S)-ol, Gal )GlcNAc( 6S)beta(1-3)Gal(6S)beta(1-4)GlcNAc(6S)-ol, and Gal(6S)beta(1-4)GlcNAc( 6S)(1 -3)Gal(6S)beta(1-4)GlcNAc(6S)-ol, where GlcNAc(6S)-ol represents N-acetyl-glucosaminitol-6-O-sulphate. The value of these data for mic rostructural analysis of undegraded keratan sulphate samples using C-1 3-NMR spectroscopy is discussed with respect to galactose sulphation l evels and the block sulphation structure.