OPEN SANDWICH ELISA - A NOVEL IMMUNOASSAY BASED ON THE INTERCHAIN INTERACTION OF ANTIBODY VARIABLE REGION

Authors
UEDA H TSUMOTO K KUBOTA K SUZUKI E NAGAMUNE T NISHIMURA H SCHUELER PA WINTER G KUMAGAI I MAHONEY WC
Citation
H. Ueda et al., OPEN SANDWICH ELISA - A NOVEL IMMUNOASSAY BASED ON THE INTERCHAIN INTERACTION OF ANTIBODY VARIABLE REGION, Nature biotechnology, 14(13), 1996, pp. 1714-1718
Citations number
35
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Nature biotechnology → ACNP
ISSN journal
10870156
Volume
14
Issue
13
Year of publication
1996
Pages
1714 - 1718
Database
ISI
SICI code
1087-0156(1996)14:13<1714:OSE-AN>2.0.ZU;2-K
Abstract
We describe an immunoassay that is based on the interchain interaction of separated V-L and V-H chains from a single chain antibody variable region. In the presence of antigen, the chains reassociate. V-L fragm ents of anti-hen egg lysozyme (HEL) antibody HyHEL-10 were immobilized on microtiter plates, Samples were coincubated with an M13-displayed V-H chain, and assayed with peroxidase-labeled anti-M13 antibody. Sign al was detected in direct proportion to the amount of HEL in the sampl e. Wide dynamic range with <15 ng/ml sensitivity was attained.