B. Malaurie et al., EFFECT OF GROWTH-REGULATORS CONCENTRATIONS ON MORPHOLOGICAL DEVELOPMENT OF MERISTEM-TIPS IN DIOSCOREA-CAYENESIS D-ROTUNDATA COMPLEX AND D-PRAEHENSILIS, Plant cell, tissue and organ culture, 41(3), 1995, pp. 229-235
The effects of NAA/BA concentrations and GA(3) supply, on the developm
ent of meristem-tips excised from microshoots of one Dioscorea cayenen
sis-D. rotundata cultivar and of one D. praehensilis genotype, have be
en examined. Three NAA/BA concentrations and one addition of GA(3) to
one medium were compared after 3, 8 and 11 months of culture without s
ubculturing. Culture support was a superimposed agar/agarose (5 v/1v);
growth regulators were used in a MS medium with agarose as top layer
support. For the two clones, rooting was significantly decreased by th
e highest NAA/BA concentrations, and budding was independent of treatm
ents. Shoot elongation was more associated with lower NAA/BA concentra
tions (Dioscorea cayenensis-D. rotundata) or independent (D. praehensi
lis). Callusing appeared more linked to higher NAA/BA concentrations (
D. praehensilis) or independent Dioscorea cayenensis-D. rotundata). GA
(3) supply did not improve regeneration. The genotypic differences are
marked by a better survival and a better regeneration into rooted pla
ntlets for the cultivar.