IN-VIVO P-31-NMR STUDIES OF AGE AND ENERGY-METABOLISM IN AN ANIMAL FLAP MODEL

Citation
Mp. Schweizer et al., IN-VIVO P-31-NMR STUDIES OF AGE AND ENERGY-METABOLISM IN AN ANIMAL FLAP MODEL, Plastic and reconstructive surgery, 96(2), 1995, pp. 429-435
Citations number
32
Categorie Soggetti
Surgery
ISSN journal
00321052
Volume
96
Issue
2
Year of publication
1995
Pages
429 - 435
Database
ISI
SICI code
0032-1052(1995)96:2<429:IPSOAA>2.0.ZU;2-U
Abstract
Changes in phosphate energy metabolism with time in a rat flap model w ere followed noninvasively with in vivo P-31-NMR. The influence of age on high-energy phosphate metabolites in perfused and ischemic ends of 3 x 10 cm dorsal flaps was noted from 30 minutes to 7 days after clos ure in 6-, 12-, and 24-month-old (n = 4, 7, and 8, respectively) male Fischer 344 rats. Phosphocreatine to inorganic phosphate ratios showed younger animals exhibiting significant returns to preinjury energy st atus in 2- and 3-mm ischemic layers. This behavior, 24 to 72 hours aft er closure, coincides with neovascularization of the flap tissue. By c ontrast, 12- and 24-month-old animals experienced statistically signif icantly lesser high-energy rebound, developing greater necrosis in the ischemic regions. Early intracellular pH lowering, indicative of lact ate production, was somewhat greater in the flaps of younger animals. The in vivo P-31-NMR methods thus provide metabolic insights into flap behavior correlating with physiologic influences of aging.