IDENTIFICATION OF A GIBBERELLIN-INDUCED GENE IN DEEP-WATER RICE USINGDIFFERENTIAL DISPLAY OF MESSENGER-RNA

Differential display of mRNA was employed to identify gibberellin (GA) -regulated genes in deepwater rice. One of the first differentially di splayed products identified was shown to be ten-fold induced after sta rt of GA treatment. The sequence of the clone shows complete amino aci d identity with histone H3, and its increased mRNA level correlates wi th the onset of DNA synthesis. We also identified a gene whose express ion pattern did not change over the course of treatment with GA and ca n be used as standard to correct for loading differences on northern b lots.