FEMTOSECOND TIME-RESOLVED VIBRATIONAL SPECTROSCOPY OF HEME-PROTEINS

Ultra-fast pulsed laser measurements in the time domain have several a dvantages over traditional frequency-domain spectroscopy. For example, very low frequency modes are more easily probed, fluorescent samples can be studied and vibrational modes that are directly coupled to chem ical reactions are revealed, This study demonstrated some of these adv antages using heme protein samples, Low-frequency modes are observed i n deoxymyoglobin (deoxy Mb), fluorescence rejection is demonstrated wi th metMb and product-state vibrational coherence is revealed with MbNO .