MICROASSAY METHOD FOR EVALUATING THE ALLERGENICITY OF THE MAJOR SOYBEAN ALLERGEN, GLY-M BD 30K, WITH MOUSE ANTISERUM AND RBL-2H3 CELLS

A micro-assay method for evaluating the allergenicity of soybean aller gen was developed by using the mouse antiserum against Gly m Bd 30K, a major soybean allergen, and RBL-2H3, a rat mucosal mast cell line, Th e antiserum against Gly m Bd 30K was prepared by subcutaneously immuni zing BALB/c mice with the allergen. The behavior by affinity-chromatog raphy and the properties against heat treatment show that the reaginic activity of the antiserum resided in the IgE antibody specific for Gl y m Bd 30K. The developed assay method is shown to be useful for simul ating IgE mediated type-I allergy and to be highly sensitive for detec ting the allergen.