POSITIONAL SPECIFICITY AND STEREOSELECTIVITY OF A LIPASE PREPARATION FROM OAT SEEDS ACTING ON 1,2,3-TRIHEXANOYLGLYCEROL

Authors
OTA Y MINESAKI T OSHIMA A
Citation
Y. Ota et al., POSITIONAL SPECIFICITY AND STEREOSELECTIVITY OF A LIPASE PREPARATION FROM OAT SEEDS ACTING ON 1,2,3-TRIHEXANOYLGLYCEROL, Bioscience, biotechnology, and biochemistry, 61(1), 1997, pp. 166-167
Citations number
13
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
1
Year of publication
1997
Pages
166 - 167
Database
ISI
SICI code
0916-8451(1997)61:1<166:PSASOA>2.0.ZU;2-M
Abstract
Oat seed lipase was extracted with 0.01 M calcium chloride solution co ntaining 0.5% Triton X-100 and precipitated with ammonium sulfate, The precipitate was dissolved in phosphate buffer at pH 6.0 and the super natant was used as the lipase preparation, The lipase was very selecti ve in the ester positions of 1.2,3-trihexanoylglycerol, hydrolyzing sn -3 most quickly, sn-1 moderately, and sn-2 hardly at all.