ISOLATION AND CHARACTERIZATION OF CHITINASE FROM A FLAKE-CHITIN DEGRADING MARINE BACTERIUM, AEROMONAS-HYDROPHILA H-2330

Authors
HIRAGA K SHOU L KITAZAWA M TAKAHASHI S SHIMADA M SATO R ODA K
Citation
K. Hiraga et al., ISOLATION AND CHARACTERIZATION OF CHITINASE FROM A FLAKE-CHITIN DEGRADING MARINE BACTERIUM, AEROMONAS-HYDROPHILA H-2330, Bioscience, biotechnology, and biochemistry, 61(1), 1997, pp. 174-176
Citations number
13
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
1
Year of publication
1997
Pages
174 - 176
Database
ISI
SICI code
0916-8451(1997)61:1<174:IACOCF>2.0.ZU;2-F
Abstract
A flake-chitin degrading marine bacterium was isolated and identified as Aeromonas hydrophila. This strain secreted five chitinases and an b eta-N-acetylglucosaminidase. The main chitinase (Chi-A) was purified a nd characterized, The optimum pH of Chi-A was 5-8, and the activity wa s inhibited by Hg2+ and Fe3+. Chi-A Has different from chitinases of o ther Aeromonas species with respect to molecular weight (62,000) and i nsensitivity to monoiodoacetate. The amino-terminal amino acid sequenc e showed extensive similarity with chitinases from Gram-negative bacte ria.