THE SEQUENCE OF A 44,420 BP FRAGMENT LOCATED ON THE LEFT ARM OF CHROMOSOME-XIV FROM SACCHAROMYCES-CEREVISIAE

We have determined the complete nucleotide sequence of a 44 420 bp DNA fragment from chromosome XIV of Saccharomyces cerevisiae. The sequenc e data revealed 23 open reading frames (ORFs) larger than 300 bp, cove ring 73 . 5% of the sequence. The ORFs N2418, N2428, N2441, N2474 and N2480 correspond to previously sequenced S. cerevisiae genes coding re spectively for the mitochondrial import protein Mas5, the nucleolar pr otein Nop2, the outer mitochondrial membrane porin Por1, the cytochrom e c oxidase polypeptide VA precursor CoxA and the yeast protein tyrosi ne phosphatase Msg5. Translation products of three other ORFs N2406, N 2411 and N2430 exhibit similarity to previously known S. cerevisiae pr oteins: the ribosomal protein YL9A, the protein Nca3 involved in the m itochondrial expression of subunits 6 and 8 of the ATP synthase and ac tin; in addition N2505 presents strong similarity to an ORF of chromos ome IX. The predicted protein products of ORFs N2417 and N2403 present similarities with domains from proteins of other organisms: the Candi da maltosa cycloheximide-resistance protein, the human interleukin enh ancer-binding factor (ILF-2). The 12 remaining ORFs show no significan t similarity to known proteins. In addition, we have detected a DNA re gion very similar to the yeast transposon Ty 1-15 of which insertion h as disrupted a tRNA(Asp) gene. The sequence has been deposited in the GenBank database with the Accession Number U12141.