A 29-CENTER-DOT-425 KB SEGMENT ON THE LEFT ARM OF YEAST CHROMOSOME-XVCONTAINS MORE THAN TWICE AS MANY UNKNOWN AS KNOWN OPEN READING FRAMES

The nucleotide sequence of a 29 . 425 kb fragment localized on the lef t arm of chromosome XV from Saccharomyces cerevisiae has been determin ed. The sequence contains 13 open reading frames (ORFs) of which four encode the known genes ADH1, COQ3, MSH2 and RCF4. Predictions are made concerning the functions of the unknown ORFs. Some of the ORFs contai n sequences similar to expressed sequence tags (EST) found in the data base made available by TIGR. In particular, the highly expressed ADH1 gene is represented in this database by no less than 20 EST sequences. Two ARS sequences and a putative functional GCN4 motif have also been detected. One ORF (O0953) containing nine putative transmembrane segm ents is similar to a hypothetical membrane protein of Arabidopsis thal iana. Characteristic features of the other ORFs include ATP/GTP bindin g sites, a fungal Zn(2)-Cys(6) binuclear centre, an endoplasmic reticu lum targeting sequence, a beta-transducin repeat signature and in two instances, good similarity to the prokaryotic lipoprotein signal pepti de motif. The sequence has been deposited in the EMBL data library und er Accession Number X83121.