HIGH-EFFICIENCY TRANSFORMATION OF BACILLUS-BREVIS BY ELECTROPORATION

Authors
OKAMOTO A KOSUGI A KOIZUMI Y YANAGIDA F UDAKA S
Citation
A. Okamoto et al., HIGH-EFFICIENCY TRANSFORMATION OF BACILLUS-BREVIS BY ELECTROPORATION, Bioscience, biotechnology, and biochemistry, 61(1), 1997, pp. 202-203
Citations number
11
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
1
Year of publication
1997
Pages
202 - 203
Database
ISI
SICI code
0916-8451(1997)61:1<202:HTOBBE>2.0.ZU;2-C
Abstract
Various conditions in the Bacillus brevis transformation by electropor ation were investigated to increase the efficiency, Competent cell vol ume and presence of polyethylene glycol 6000 and single stranded DNA o n pulsing were critical for the efficiency, As a result, we establishe d the improved method by which 10(7)-10(8) transformants/mu g plasmid DNA could be obtained.