A NOVEL PROTEIN, AMYLOID PRECURSOR-LIKE PROTEIN-2, IS PRESENT IN HUMAN BRAIN, CEREBROSPINAL-FLUID AND CONDITIONED MEDIA

Authors
WEBSTER MT GROOME N FRANCIS PT PEARCE BR SHERRIFF FE THINAKARAN G FELSENSTEIN KM WASCO W TANZI RE BOWEN DM
Citation
Mt. Webster et al., A NOVEL PROTEIN, AMYLOID PRECURSOR-LIKE PROTEIN-2, IS PRESENT IN HUMAN BRAIN, CEREBROSPINAL-FLUID AND CONDITIONED MEDIA, Biochemical journal, 310, 1995, pp. 95-99
Citations number
25
Categorie Soggetti
Biology
Journal title
Biochemical journal → ACNP
ISSN journal
02646021
Volume
310
Year of publication
1995
Part
1
Pages
95 - 99
Database
ISI
SICI code
0264-6021(1995)310:<95:ANPAPP>2.0.ZU;2-A
Abstract
A monoclonal antibody, 3B11, was raised to a novel protein, amyloid pr ecursor-like protein 2, which did not recognize amyloid precursor prot ein. Multiple bands were detected in human brain fractions and cell ly sate by Western blotting, indicating the presence of isoforms. 3B11 im munoreactivity was also detected in cerebrospinal fluid and conditione d medium, indicating that the protein is secreted. Immunocytochemistry revealed 3B11 immunoreactivity in sections of human brain.