ENDOPLASMIC-RETICULUM AS A STORAGE SITE FOR ALLERGENIC PROTEINS IN POLLEN GRAINS OF SEVERAL OLEACEAE

Citation
Mi. Rodriguezgarcia et al., ENDOPLASMIC-RETICULUM AS A STORAGE SITE FOR ALLERGENIC PROTEINS IN POLLEN GRAINS OF SEVERAL OLEACEAE, Protoplasma, 187(1-4), 1995, pp. 111-116
Citations number
28
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
0033183X
Volume
187
Issue
1-4
Year of publication
1995
Pages
111 - 116
Database
ISI
SICI code
0033-183X(1995)187:1-4<111:EAASSF>2.0.ZU;2-4
Abstract
The ultrastructure of mature pollen grains of several Oleaceae species (Olea europaea, Fraxinus excelsior, Syringa vulgaris, Ligustrum vulga re, and Forsythia suspensa) was studied and the immunolocalization of Ole e I, the major allergen of olige pollen, was determined by immunog old labelling. The five Oleaceae pollens studied here showed different intensities of labelling. The Ole e I allergen was localized througho ut the rough endoplasmic reticulum. The absence of gold particles in o ther cell compartments, such as nuclei, pastids, mitochondria, dictyos omes, lipid bodies, and cell wall, as well as the absence of labelling in control preparations, indicate the specificity of immunolocalizati on. We conclude that endoplasmic reticulum of the mature pollen grain is a storage site for allergenic proteins and is probably also involve d in their synthesis.