Dg. Kennedy et al., DEVELOPMENT OF AN ELISA FOR MADURAMICIN AND DETERMINATION OF THE DEPLETION KINETICS OF MADURAMICIN RESIDUES IN POULTRY, Food additives and contaminants, 14(1), 1997, pp. 27-33
Polyclonal antibodies against the coccidiostat maduramicin were produc
ed by the immunization of rabbits with a conjugate between maduramicin
and human serum albumin. The antibodies exhibited minimal cross-react
ivity with structurally similar ionaphoric coccidiostats (monensin, sa
linomycin, narasin and laslocid). An enzyme-linked immunosorbent assay
(ELISA) was developed for maduramicin in poultry tissue using these a
ntibodies. The ELISA had a limit of detection of approximately 0.02 ng
/g in tissue (ng/ml in serum) and a limit of determination of 1.0 ng/g
in tissue (ng/ml in serum). The half-life of maduramicin was measured
in broiler chickens that had been treated with a therapeutic dose of
maduramicin for 14 days. Following withdrawal of the medicated feed, t
he mean half-lives of maduramicin were 20, 39 and 13 h in liver, muscl
e and serum, respectively. At the recommended withdrawal period (5 day
s) the mean residual maduramicin concentration in liver was 1.6 ng/g,
in muscle was 1.5 ng/g and in serum was 0.05 ng/ml.