Azodicarbonamide (ADA), a dough conditioner, is an additive approved i
n the US up to a maximum of 45 mg/kg in flour. The addition of 45 mg/k
g of ADA was investigated and found to increase the ethyl carbamate (E
C) content of commercially prepared bread; by 1-3 mu g/kg. A similar i
ncrease in EC was observed in breads baked in the laboratory with a br
ead machine. The increase in EC levels appears to depend on a variety
of factors, most notably the concentration of ADA added and the time o
f fermentation. The addition of 20 mg/kg ADA caused only a slight incr
ease, if any, in commercial products but a 2.3 mu g/kg increase of EC
in breads baked with a bread machine. When 100 mg/kg of ascorbic acid
was added along with ADA, smaller EC increases were observed. Addition
of urea was also found to enhance the BC content of the bread. Toasti
ng, which was previously shown to increase EC levels, caused even larg
er increases when ADA or urea had been added.