PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST THE RECOMBINANT P50(CSK) PROTEIN-TYROSINE KINASE - A TOOL FOR SIGNAL-TRANSDUCTION RESEARCH

p50(csk) is a protein tyrosine kinase (PTK) that has been reported to regulate the activity of other PTKs belonging to the src gene family, Several hybridoma clones that produce monoclonal antibodies (MAbs) dir ected against recombinant p50(csk) were established, Five of the clone s were analyzed for their ability to recognize native and denatured p5 0(csk) protein after undergoing native and denaturing polyacrylamide g el electrophoresis followed by western blotting, In addition, the clon es were tested for their ability to immunoprecipitate p50(csk) and yet maintain tyrosine kinase activity of antibody-bound p50(csk), None of the clones cross-reacted with pp60(c-src), a PTK that shares with p50 (csk) the homologous SH1 catalytic domain and SH2 and SH3 regulatory d omains, These MAbs can be used to study p50(csk) directly, and its rol e in regulating members of the src family.