HUMAN BLEED PLATELET PROTEIN MAP ESTABLISHED BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS

Two-dimensional (2-D) maps of cytosol and enriched-membrane platelet p roteins has allowed the identification of more than 25 spots by three different methods: matching of the platelet gels with other 2-D refere nce maps, immunoblotting with chemiluminescence detection, and N-termi nal sequencing. Different G protein (guanosine triphosphate-binding pr otein) subunits, cytoskeletal proteins, and proteins common to the hum an liver, red blood cells and plasma were identified. The two platelet protein maps presented here contribute to the project of identificati on of human cell and body fluid proteins. They may serve as working to ols since platelets are popular models for the study of central nervou s system neurotransmitter systems and stimulus-response coupling mecha nisms.