CLONING AND CHARACTERIZATION OF A CDNA-ENCODING A CELLOBIOSE DEHYDROGENASE FROM THE WHITE-ROT FUNGUS PHANEROCHAETE-CHRYSOSPORIUM

The cDNA of cellobiose dehydrogenase (CDH) from Phanerochaete chrysosp orium has been cloned and sequenced. The 5' end was obtained by PCR am plification. The cDNA contains 2310 translated bases excluding the pol y(A) tail. The deduced mature protein contains 770 amino acid residues and is preceded by a 18 residue long signal peptide. The regions of t he amino acid sequence corresponding to the heme and FAD domains of CD H were identified as well as the nucleotide-binding motif, the disulfi de pairing and a methionine residue chelating the heme iron. No homolo gous sequences mere found for the heme domain, however, the FAD domain appears to be distantly related to the GMC oxidoreductase family.