MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF HUMAN PREPROCATHEPSIN-C

Authors
PARIS A STRUKELJ B PUNGERCAR J RENKO M DOLENC I TURK V
Citation
A. Paris et al., MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF HUMAN PREPROCATHEPSIN-C, FEBS letters, 369(2-3), 1995, pp. 326-330
Citations number
30
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
369
Issue
2-3
Year of publication
1995
Pages
326 - 330
Database
ISI
SICI code
0014-5793(1995)369:2-3<326:MASOHP>2.0.ZU;2-E
Abstract
A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney-derived RT-PCR probe an d its complete nucleotide sequence determined. The full-length 1857 bp sequence codes for a protein of 463 amino acid residues with a calcul ated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5% identit y. A multiple alignment of the deduced cathepsin C sequence of 233 res idues which, by analogy to other cysteine proteinases, corresponds to the mature protein, confirms that human cathepsin C belongs to tbe pap ain superfamily.