THE FIRST UNTRANSLATED EXON OF THE HUMAN TENASCIN-C GENE PLAYS A REGULATORY ROLE IN GENE-TRANSCRIPTION

Authors
GHERZI R PONASSI M GAGGERO B ZARDI L
Citation
R. Gherzi et al., THE FIRST UNTRANSLATED EXON OF THE HUMAN TENASCIN-C GENE PLAYS A REGULATORY ROLE IN GENE-TRANSCRIPTION, FEBS letters, 369(2-3), 1995, pp. 335-339
Citations number
28
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
369
Issue
2-3
Year of publication
1995
Pages
335 - 339
Database
ISI
SICI code
0014-5793(1995)369:2-3<335:TFUEOT>2.0.ZU;2-#
Abstract
The transcription of the human tenacin-C (TN-C) gene is directed by a single promoter. Here we demonstrate, in transiently transfected cells , that two distinct regions of the untranslated 179 bp-long exon 1 pla y antagonistic roles in transcriptional regulation: bases from 1 to 20 strongly increase the transcription of the reporter gene CAT directed by the human TN-C gene promoter, while bases from 79 to 179 significa ntly reduce this activation.