Many in vitro studies have described the minimal thermal damage associ
ated with the erbium-YAG laser during preparation of cuts in bone. How
ever, not all of the in vivo studies have shown improved healing of la
sed bone over the more conventional methods of bone surgery. In this i
n vivo study, 26 mature rats were used. Under general anaesthesia, a s
lot was produced in the tail bones of each rat using the erbium-YAG la
ser and a conventional burr. The rats were killed at intervals over a
24-day period, and the tail bones collected in neutral buffered formal
in, decalcified and prepared for routine wax histology. The main histo
logical differences occurred at 9 and 10 days after surgery, when the
bone trabeculae formed following laser surgery appeared to extend into
the marrow from the cortical defect in long, narrow finger-like proje
ctions. The migration of fibroblasts from the periosteum following las
er surgery, and their subsequent differentiation into osteoblasts in t
he marrow may be responsible for this histological pattern. Surgery wi
th the burr produced a coarse trabecular pattern limited to the outer
cortical region.