DIETARY CORRECTION OF HYPERCHOLESTEROLEMIA IN THE RABBIT NORMALIZES ENDOTHELIAL SUPEROXIDE ANION PRODUCTION

Background We have shown that hypercholesterolemia increases vascular superoxide anion (O-2(-)) production, which could be responsible for a ugmented inactivation of endothelium-derived vascular relaxing factor. We sought to determine whether this increased vascular O-2(-) product ion is due to infiltration of macrophages into the intima and whether dietary treatment of hypercholesterolemia normalizes O-2(-) production . Methods and Results A specific and sensitive assay for O-2(-) based on chemiluminescence of lucigenin was used; the amount of O-2(-) produ ced by vascular ring segments was quantified based on known quantities of O-2(-) produced by xanthine-xanthine oxidase standards. O-2(-) pro duction of aortic segments from normal rabbits (n=9), cholesterol-fed rabbits (1% cholesterol diet for 1 month, n=7), and rabbits fed a 1% c holesterol diet for 1 month followed by a normal diet for 1 month (reg ression rabbits, n=5) was measured. At the end of these diets, serum c holesterol levels were 1.5+/-0.2, 26.0+/-3.9, and 1.8+/-0.5 mmol/L (58 +/-6, 1000+/-150, and 71+/-19 mg/dL) in the normal, cholesterol-fed, a nd regression animals, respectively. Vessels from normal rabbits with endothelium produced 0.32+/-0.06 nmol O-2(-)/mg dry wt per minute, whe reas those without endothelium produced approximately twice as much O- 2(-) (0.66+/-0.12 nmol O-2(-) mg dry wt per minute. Vessels with endot helium from cholesterol-fed rabbits produced 4.5-fold more O-2(-) than vessels from normal animals. This increased production of O-2(-) was normalized by endothelial removal. This increased production of O-2(-) was not due to infiltration of macrophages in the intima, because the re was no correlation between vascular O-2(-) production and macrophag e infiltration assessed by immunohistochemistry with use of a specific antibody against rabbit macrophage. O-2(-) production by vessels from regression rabbits was similar to that observed in normal animals, an d as in the normal rabbits, endothelial removal increased O-2(-) produ ction. Aortic rings from these animals also were studied in organ cham bers. Dietary lowering of cholesterol dramatically improved vasodilato r responses to acetylcholine and A23187 (P<.05 versus cholesterol-fed rabbits). Conclusions Dietary lowering of cholesterol not only improve s endothelium-dependent vascular relaxation but also normalizes endoth elial O-2(-) production. Decreases of O-2(-) production by dietary low ering of cholesterol not only may improve vasomotor control but also m ay improve other aspects of vascular integrity in atherosclerosis.