A 67-KDA PROTEIN-BINDING TO THE CURVED DNA IN THE MAIN REGULATORY REGION OF THE RAT MITOCHONDRIAL GENOME

We have purified, by sequence-specific affinity chromatography, a mito chondrial (mt) matrix protein which binds to the curved DNA located be tween the replication origin (ori) of the leading strand (ori-H) and t he two transcription promoters in the rat mt genome. The protein was c haracterized by gel electrophoresis as a 67-kDa polypeptide and seems to be involved in the DNA contact on the mt light strand. This protein differs (in the size and location of its DNA-binding site) from other DNA-binding proteins studied so far in animal mt systems. We suggest a role for the 67-kDa protein, assisted by other proteins, in regulati ng the initiation of leading-strand replication.