CLONING OF THE CDNA-ENCODING NEURAL ADHESION MOLECULE F3 FROM BOVINE BRAIN

We cloned a bovine cDNA encoding the neural adhesion molecule F3 and a nalyzed its nucleotide sequence. The coding region consisted of 3054 b p encoding 1018 amino acid (aa) residues. The M(r) calculated from the deduced aa sequence was 113 383. Bovine F3 had 93, 94 and 77% aa iden tity with the mouse, human and chicken homologs, respectively. Bovine F3, similar to those of chicken and human, was devoid of two aa residu es (Ile-Thr) in the sixth immunoglobulin type C2-like domain, as compa red with the mouse homolog. Parts of bovine F3 protein were overproduc ed in Escherichia coli. The antibodies raised against the recombinant proteins in rabbits reacted specifically with F3. F3 protein was detec ted in cerebellum cerebrum and spinal cord in Western blot analysis.