Ma. Stamnes et al., AN INTEGRAL MEMBRANE COMPONENT OF COATOMER-COATED TRANSPORT VESICLES DEFINES A FAMILY OF PROTEINS INVOLVED IN BUDDING, Proceedings of the National Academy of Sciences of the United Statesof America, 92(17), 1995, pp. 8011-8015
We have isolated a major integral membrane protein from Golgi derived
coatomer-coated vesicles, This 24-kDa protein, p24, defines a family o
f integral membrane proteins with homologs present in yeast and humans
, In addition to sequence similarity, all p24 family members contain a
motif with the characteristic heptad repeats found in coiled coils, W
hen the yeast p24 isoform, yp24A, is knocked out in a strain defective
for vesicle fusion, a dramatic reduction in the accumulation of trans
port vesicles is observed, Together, these results indicate a role for
this protein family in the budding of coatamer-coated and other speci
es of coated vesicles.