T. Wagner et al., PH-DEPENDENT DENATURATION OF EXTRACELLULAR ASPARTIC PROTEINASES FROM CANDIDA SPECIES, Journal of medical and veterinary mycology, 33(4), 1995, pp. 275-278
The yeasts Candida albicans, Candida tropicalis, and Candida parapsilo
sis secrete aspartic proteinases which may enhance virulence. Profiles
of pH-dependent irreversible denaturation of such enzymes were determ
ined at 37 degrees C. C. albicans proteinases from both serotypes A an
d B maintained 50% of their activity near pH 7.25. Proteinases from C.
parapsilosis and C. tropicalis lost 50% of their activity at pH 6.75
and pH 6.15, respectively. This suggests that in the infected host onl
y proteinases of C. albicans maintain a native state for any length of
time.