DETECTION OF SHORT-CHAIN ALPHA-HYDROXYALDEHYDIC COMPOUNDS AS PENTAFLUORBENZYLOXIME DERIVATIVES IN BOVINE LIVER

Pentafluorbenzyloxime derivatization allows fast, gentle and unambiguo us identification of alpha-hydroxyaldehydic lipid peroxidation product s via GC/MS in biological material. Even 1.5 g of a bovine liver sampl e is sufficient to detect short-chain 2-hydroxyalkanales resulting fro m cleavage reactions of dioxygenated fatty acids. Quantification is ac hieved after secondary derivatization with N-methyl-N-t-butyldimethyls ilyltrifluoracetamide (M-t-BSTFA) by mass spectrometry using character istic ion traces of the derivatives. In addition, the corresponding (n -1)-hydroxy-n-oxo acids, previously unknown in biological material, co uld be detected.