A. Loidlstahlhofen et al., DETECTION OF SHORT-CHAIN ALPHA-HYDROXYALDEHYDIC COMPOUNDS AS PENTAFLUORBENZYLOXIME DERIVATIVES IN BOVINE LIVER, Chemistry and physics of lipids, 77(1), 1995, pp. 113-119
Pentafluorbenzyloxime derivatization allows fast, gentle and unambiguo
us identification of alpha-hydroxyaldehydic lipid peroxidation product
s via GC/MS in biological material. Even 1.5 g of a bovine liver sampl
e is sufficient to detect short-chain 2-hydroxyalkanales resulting fro
m cleavage reactions of dioxygenated fatty acids. Quantification is ac
hieved after secondary derivatization with N-methyl-N-t-butyldimethyls
ilyltrifluoracetamide (M-t-BSTFA) by mass spectrometry using character
istic ion traces of the derivatives. In addition, the corresponding (n
-1)-hydroxy-n-oxo acids, previously unknown in biological material, co
uld be detected.